Construct: shRNA TRCN0000123740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172592.1-2039s1c1
DNA Barcode:
CGAAGGTAAAGTACAGCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srek1 (218543)
Gene Description:
splicing regulatory glutamine/lysine-rich protein 1
Transcript:
RefSeq NM_172592.1 (NON-CURRENT)
Match location:
Position 2039 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGGTAAAGTACAGCACAA
Hairpin Sequence:
5'-CCGG-CGAAGGTAAAGTACAGCACAA-CTCGAG-TTGTGCTGTACTTTACCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGGTAAAGTACAGCACAACTCGAGTTGTGCTGTACTTTACCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGGTAAAGTACAGCACAACTCGAGTTGTGCTGTACTTTACCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000287194