Construct: shRNA TRCN0000123742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172592.1-876s1c1
DNA Barcode:
GCTGATCAACTACTTGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srek1 (218543)
Gene Description:
splicing regulatory glutamine/lysine-rich protein 1
Transcript:
RefSeq NM_172592.1 (NON-CURRENT)
Match location:
Position 876 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATCAACTACTTGAATTT
Hairpin Sequence:
5'-CCGG-GCTGATCAACTACTTGAATTT-CTCGAG-AAATTCAAGTAGTTGATCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATCAACTACTTGAATTTCTCGAGAAATTCAAGTAGTTGATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATCAACTACTTGAATTTCTCGAGAAATTCAAGTAGTTGATCAGC-3'

Other clones with same target sequence:

  1. TRCN0000298293