Construct: shRNA TRCN0000123853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029528.1-607s1c1
DNA Barcode:
CTACACTTATGGCTTGGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam131b (76156)
Gene Description:
family with sequence similarity 131, member B
Transcript:
RefSeq NM_029528.1 (NON-CURRENT)
Match location:
Position 607 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACACTTATGGCTTGGTCTT
Hairpin Sequence:
5'-CCGG-CTACACTTATGGCTTGGTCTT-CTCGAG-AAGACCAAGCCATAAGTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACACTTATGGCTTGGTCTTCTCGAGAAGACCAAGCCATAAGTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACACTTATGGCTTGGTCTTCTCGAGAAGACCAAGCCATAAGTGTAG-3'

Other clones with same target sequence:

(none)