Construct: shRNA TRCN0000123868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026760.1-176s1c1
DNA Barcode:
TCCAGCGGTGTGAACTTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trir (68544)
Gene Description:
telomerase RNA component interacting RNase
Transcript:
RefSeq NM_026760.1 (NON-CURRENT)
Match location:
Position 176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGCGGTGTGAACTTGTTT
Hairpin Sequence:
5'-CCGG-TCCAGCGGTGTGAACTTGTTT-CTCGAG-AAACAAGTTCACACCGCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGCGGTGTGAACTTGTTTCTCGAGAAACAAGTTCACACCGCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGCGGTGTGAACTTGTTTCTCGAGAAACAAGTTCACACCGCTGGA-3'

Other clones with same target sequence:

  1. TRCN0000354174