Construct: shRNA TRCN0000123912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028007.1-1332s1c1
DNA Barcode:
GCAGATGCTTACTTTGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itfg1 (71927)
Gene Description:
integrin alpha FG-GAP repeat containing 1
Transcript:
RefSeq NM_028007.1 (NON-CURRENT)
Match location:
Position 1332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGCTTACTTTGTTAAA
Hairpin Sequence:
5'-CCGG-GCAGATGCTTACTTTGTTAAA-CTCGAG-TTTAACAAAGTAAGCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGCTTACTTTGTTAAACTCGAGTTTAACAAAGTAAGCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGCTTACTTTGTTAAACTCGAGTTTAACAAAGTAAGCATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000317265