Construct: shRNA TRCN0000123922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012322.1-694s1c1
DNA Barcode:
GTCAACATGAATGGCTCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sctr (319229)
Gene Description:
secretin receptor
Transcript:
RefSeq NM_001012322.1 (NON-CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAACATGAATGGCTCCTTT
Hairpin Sequence:
5'-CCGG-GTCAACATGAATGGCTCCTTT-CTCGAG-AAAGGAGCCATTCATGTTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAACATGAATGGCTCCTTTCTCGAGAAAGGAGCCATTCATGTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAACATGAATGGCTCCTTTCTCGAGAAAGGAGCCATTCATGTTGAC-3'

Other clones with same target sequence:

(none)