Construct: shRNA TRCN0000123935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024385.1-730s1c1
DNA Barcode:
CCCACTCACTTACATGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crls1 (66586)
Gene Description:
cardiolipin synthase 1
Transcript:
RefSeq NM_001024385.1 (CURRENT)
Match location:
Position 730 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTCACTTACATGATAAT
Hairpin Sequence:
5'-CCGG-CCCACTCACTTACATGATAAT-CTCGAG-ATTATCATGTAAGTGAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTCACTTACATGATAATCTCGAGATTATCATGTAAGTGAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTCACTTACATGATAATCTCGAGATTATCATGTAAGTGAGTGGG-3'

Other clones with same target sequence:

(none)