Construct: shRNA TRCN0000123954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145835.1-1987s1c1
DNA Barcode:
CTCTCCACCTTCATCTGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lctl (235435)
Gene Description:
lactase-like
Transcript:
RefSeq NM_145835.1 (NON-CURRENT)
Match location:
Position 1987 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCCACCTTCATCTGTCAA
Hairpin Sequence:
5'-CCGG-CTCTCCACCTTCATCTGTCAA-CTCGAG-TTGACAGATGAAGGTGGAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCCACCTTCATCTGTCAACTCGAGTTGACAGATGAAGGTGGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCCACCTTCATCTGTCAACTCGAGTTGACAGATGAAGGTGGAGAG-3'

Other clones with same target sequence:

(none)