Construct: shRNA TRCN0000123977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021314.3-2376s1c1
DNA Barcode:
CCCTTGTGAATGCAGCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tacc2 (57752)
Gene Description:
transforming, acidic coiled-coil containing protein 2
Transcript:
RefSeq NM_021314.3 (NON-CURRENT)
Match location:
Position 2376 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGTGAATGCAGCAACAA
Hairpin Sequence:
5'-CCGG-CCCTTGTGAATGCAGCAACAA-CTCGAG-TTGTTGCTGCATTCACAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGTGAATGCAGCAACAACTCGAGTTGTTGCTGCATTCACAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGTGAATGCAGCAACAACTCGAGTTGTTGCTGCATTCACAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000323561