Construct: shRNA TRCN0000124035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201232.1-5657s1c1
DNA Barcode:
GCGTGCTTGATTGTTCGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nipbl (71175)
Gene Description:
Nipped-B homolog (Drosophila)
Transcript:
RefSeq NM_201232.1 (NON-CURRENT)
Match location:
Position 5657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGCTTGATTGTTCGATAT
Hairpin Sequence:
5'-CCGG-GCGTGCTTGATTGTTCGATAT-CTCGAG-ATATCGAACAATCAAGCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGCTTGATTGTTCGATATCTCGAGATATCGAACAATCAAGCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGCTTGATTGTTCGATATCTCGAGATATCGAACAATCAAGCACGC-3'

Other clones with same target sequence:

  1. TRCN0000317135