Construct: shRNA TRCN0000124042

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144792.3-1535s1c1
DNA Barcode:
CCACCTTAGTAGGCAAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgms1 (208449)
Gene Description:
sphingomyelin synthase 1
Transcript:
RefSeq NM_144792.3 (NON-CURRENT)
Match location:
Position 1535 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124042)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTTAGTAGGCAAGTTAA
Hairpin Sequence:
5'-CCGG-CCACCTTAGTAGGCAAGTTAA-CTCGAG-TTAACTTGCCTACTAAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTTAGTAGGCAAGTTAACTCGAGTTAACTTGCCTACTAAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTTAGTAGGCAAGTTAACTCGAGTTAACTTGCCTACTAAGGTGG-3'

Other clones with same target sequence:

(none)