Construct: shRNA TRCN0000124085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001493.2-85s1c1
DNA Barcode:
CCCAACAAAGTGCTGAGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr83os (414077)
Gene Description:
WD repeat domain 83 opposite strand
Transcript:
RefSeq NM_001001493.2 (CURRENT)
Match location:
Position 85 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACAAAGTGCTGAGGTAT
Hairpin Sequence:
5'-CCGG-CCCAACAAAGTGCTGAGGTAT-CTCGAG-ATACCTCAGCACTTTGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACAAAGTGCTGAGGTATCTCGAGATACCTCAGCACTTTGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACAAAGTGCTGAGGTATCTCGAGATACCTCAGCACTTTGTTGGG-3'

Other clones with same target sequence:

(none)