Construct: shRNA TRCN0000124104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011236.1-1471s1c1
DNA Barcode:
GCTGAACTCTTTAGAGGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad52 (19365)
Gene Description:
RAD52 homolog, DNA repair protein
Transcript:
RefSeq NM_011236.1 (NON-CURRENT)
Match location:
Position 1471 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACTCTTTAGAGGACTA
Hairpin Sequence:
5'-CCGG-GCTGAACTCTTTAGAGGACTA-CTCGAG-TAGTCCTCTAAAGAGTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACTCTTTAGAGGACTACTCGAGTAGTCCTCTAAAGAGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACTCTTTAGAGGACTACTCGAGTAGTCCTCTAAAGAGTTCAGC-3'

Other clones with same target sequence:

(none)