Construct: shRNA TRCN0000124189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172507.2-3233s1c1
DNA Barcode:
CCCAGTATTTACATTTCCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3bgrl2 (212531)
Gene Description:
SH3 domain binding glutamic acid-rich protein like 2
Transcript:
RefSeq NM_172507.2 (NON-CURRENT)
Match location:
Position 3233 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTATTTACATTTCCATA
Hairpin Sequence:
5'-CCGG-CCCAGTATTTACATTTCCATA-CTCGAG-TATGGAAATGTAAATACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTATTTACATTTCCATACTCGAGTATGGAAATGTAAATACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTATTTACATTTCCATACTCGAGTATGGAAATGTAAATACTGGG-3'

Other clones with same target sequence:

  1. TRCN0000331840