Construct: shRNA TRCN0000124205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016926.1-996s1c1
DNA Barcode:
GCCCGTATTCAGTTGATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sart3 (53890)
Gene Description:
squamous cell carcinoma antigen recognized by T cells 3
Transcript:
RefSeq NM_016926.1 (CURRENT)
Match location:
Position 996 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTATTCAGTTGATCTTT
Hairpin Sequence:
5'-CCGG-GCCCGTATTCAGTTGATCTTT-CTCGAG-AAAGATCAACTGAATACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTATTCAGTTGATCTTTCTCGAGAAAGATCAACTGAATACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTATTCAGTTGATCTTTCTCGAGAAAGATCAACTGAATACGGGC-3'

Other clones with same target sequence:

  1. TRCN0000302498