Construct: shRNA TRCN0000124216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148927.1-884s1c1
DNA Barcode:
GCATACTCCTTTGAGTCGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekha4 (69217)
Gene Description:
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
Transcript:
RefSeq NM_148927.1 (NON-CURRENT)
Match location:
Position 884 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATACTCCTTTGAGTCGCAT
Hairpin Sequence:
5'-CCGG-GCATACTCCTTTGAGTCGCAT-CTCGAG-ATGCGACTCAAAGGAGTATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATACTCCTTTGAGTCGCATCTCGAGATGCGACTCAAAGGAGTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATACTCCTTTGAGTCGCATCTCGAGATGCGACTCAAAGGAGTATGC-3'

Other clones with same target sequence:

(none)