Construct: shRNA TRCN0000124309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153578.1-1419s1c1
DNA Barcode:
CGGTATGTGAATCGAGTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nipa1 (233280)
Gene Description:
non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human)
Transcript:
RefSeq NM_153578.1 (NON-CURRENT)
Match location:
Position 1419 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTATGTGAATCGAGTTGTT
Hairpin Sequence:
5'-CCGG-CGGTATGTGAATCGAGTTGTT-CTCGAG-AACAACTCGATTCACATACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTATGTGAATCGAGTTGTTCTCGAGAACAACTCGATTCACATACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTATGTGAATCGAGTTGTTCTCGAGAACAACTCGATTCACATACCG-3'

Other clones with same target sequence:

(none)