Construct: shRNA TRCN0000124315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207675.2-921s1c1
DNA Barcode:
CCTGTTCATCAATAACCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cadm1 (54725)
Gene Description:
cell adhesion molecule 1
Transcript:
RefSeq NM_207675.2 (CURRENT)
Match location:
Position 921 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTCATCAATAACCTAAA
Hairpin Sequence:
5'-CCGG-CCTGTTCATCAATAACCTAAA-CTCGAG-TTTAGGTTATTGATGAACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTCATCAATAACCTAAACTCGAGTTTAGGTTATTGATGAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTCATCAATAACCTAAACTCGAGTTTAGGTTATTGATGAACAGG-3'

Other clones with same target sequence:

  1. TRCN0000317433