Construct: shRNA TRCN0000124363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026137.2-974s1c1
DNA Barcode:
CATGAACATCTCCACAGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr13 (73447)
Gene Description:
WD repeat domain 13
Transcript:
RefSeq NM_026137.2 (NON-CURRENT)
Match location:
Position 974 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAACATCTCCACAGGCAA
Hairpin Sequence:
5'-CCGG-CATGAACATCTCCACAGGCAA-CTCGAG-TTGCCTGTGGAGATGTTCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAACATCTCCACAGGCAACTCGAGTTGCCTGTGGAGATGTTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAACATCTCCACAGGCAACTCGAGTTGCCTGTGGAGATGTTCATG-3'

Other clones with same target sequence:

  1. TRCN0000349525