Construct: shRNA TRCN0000124438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023625.2-1171s1c1
DNA Barcode:
CCAGTGGATGATTGTGGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plbd2 (71772)
Gene Description:
phospholipase B domain containing 2
Transcript:
RefSeq NM_023625.2 (NON-CURRENT)
Match location:
Position 1171 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGGATGATTGTGGACTA
Hairpin Sequence:
5'-CCGG-CCAGTGGATGATTGTGGACTA-CTCGAG-TAGTCCACAATCATCCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGGATGATTGTGGACTACTCGAGTAGTCCACAATCATCCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGGATGATTGTGGACTACTCGAGTAGTCCACAATCATCCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000309874