Construct: shRNA TRCN0000124478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007583.1-1127s1c1
DNA Barcode:
CGTTGATCTACAATCACTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Best3 (382427)
Gene Description:
bestrophin 3
Transcript:
RefSeq NM_001007583.1 (CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGATCTACAATCACTGAT
Hairpin Sequence:
5'-CCGG-CGTTGATCTACAATCACTGAT-CTCGAG-ATCAGTGATTGTAGATCAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGATCTACAATCACTGATCTCGAGATCAGTGATTGTAGATCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGATCTACAATCACTGATCTCGAGATCAGTGATTGTAGATCAACG-3'

Other clones with same target sequence:

(none)