Construct: shRNA TRCN0000124514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018772.2-769s1c1
DNA Barcode:
CATACCATTGTGTAGCTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bri3 (55950)
Gene Description:
brain protein I3
Transcript:
RefSeq NM_018772.2 (NON-CURRENT)
Match location:
Position 769 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACCATTGTGTAGCTTGTT
Hairpin Sequence:
5'-CCGG-CATACCATTGTGTAGCTTGTT-CTCGAG-AACAAGCTACACAATGGTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACCATTGTGTAGCTTGTTCTCGAGAACAAGCTACACAATGGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACCATTGTGTAGCTTGTTCTCGAGAACAAGCTACACAATGGTATG-3'

Other clones with same target sequence:

(none)