Construct: shRNA TRCN0000124573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033509.2-580s1c1
DNA Barcode:
TGGAGATAAATCAGTGACGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vangl2 (93840)
Gene Description:
vang-like 2 (van gogh, Drosophila)
Transcript:
RefSeq NM_033509.2 (NON-CURRENT)
Match location:
Position 580 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGATAAATCAGTGACGAT
Hairpin Sequence:
5'-CCGG-TGGAGATAAATCAGTGACGAT-CTCGAG-ATCGTCACTGATTTATCTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGATAAATCAGTGACGATCTCGAGATCGTCACTGATTTATCTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGATAAATCAGTGACGATCTCGAGATCGTCACTGATTTATCTCCA-3'

Other clones with same target sequence:

(none)