Construct: shRNA TRCN0000124620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023529.1-243s1c1
DNA Barcode:
CTACAGAAGTTGGGTGGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ms4a10 (69826)
Gene Description:
membrane-spanning 4-domains, subfamily A, member 10
Transcript:
RefSeq NM_023529.1 (NON-CURRENT)
Match location:
Position 243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAGAAGTTGGGTGGCTTT
Hairpin Sequence:
5'-CCGG-CTACAGAAGTTGGGTGGCTTT-CTCGAG-AAAGCCACCCAACTTCTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAGAAGTTGGGTGGCTTTCTCGAGAAAGCCACCCAACTTCTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAGAAGTTGGGTGGCTTTCTCGAGAAAGCCACCCAACTTCTGTAG-3'

Other clones with same target sequence:

(none)