Construct: shRNA TRCN0000124621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023529.1-705s1c1
DNA Barcode:
GACACACCTATGGAACTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ms4a10 (69826)
Gene Description:
membrane-spanning 4-domains, subfamily A, member 10
Transcript:
RefSeq NM_023529.1 (NON-CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACACACCTATGGAACTTAAA
Hairpin Sequence:
5'-CCGG-GACACACCTATGGAACTTAAA-CTCGAG-TTTAAGTTCCATAGGTGTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACACACCTATGGAACTTAAACTCGAGTTTAAGTTCCATAGGTGTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACACACCTATGGAACTTAAACTCGAGTTTAAGTTCCATAGGTGTGTC-3'

Other clones with same target sequence:

(none)