Construct: shRNA TRCN0000124700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007704.2-1889s1c1
DNA Barcode:
CCCATATTTATTGCCATGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Patj (12695)
Gene Description:
PATJ, crumbs cell polarity complex component
Transcript:
RefSeq NM_007704.2 (CURRENT)
Match location:
Position 1889 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATATTTATTGCCATGATT
Hairpin Sequence:
5'-CCGG-CCCATATTTATTGCCATGATT-CTCGAG-AATCATGGCAATAAATATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATATTTATTGCCATGATTCTCGAGAATCATGGCAATAAATATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATATTTATTGCCATGATTCTCGAGAATCATGGCAATAAATATGGG-3'

Other clones with same target sequence:

(none)