Construct: shRNA TRCN0000124704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031772.1-4350s1c1
DNA Barcode:
CCTCCCAAATATAGGATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lin28b (380669)
Gene Description:
lin-28 homolog B (C. elegans)
Transcript:
RefSeq NM_001031772.1 (NON-CURRENT)
Match location:
Position 4350 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCAAATATAGGATGTTT
Hairpin Sequence:
5'-CCGG-CCTCCCAAATATAGGATGTTT-CTCGAG-AAACATCCTATATTTGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCAAATATAGGATGTTTCTCGAGAAACATCCTATATTTGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCAAATATAGGATGTTTCTCGAGAAACATCCTATATTTGGGAGG-3'

Other clones with same target sequence:

(none)