Construct: shRNA TRCN0000124787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011677.1-308s1c1
DNA Barcode:
CGTCAAGCTAATGGGATTTGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ung (22256)
Gene Description:
uracil DNA glycosylase
Transcript:
RefSeq NM_011677.1 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAAGCTAATGGGATTTGT
Hairpin Sequence:
5'-CCGG-CGTCAAGCTAATGGGATTTGT-CTCGAG-ACAAATCCCATTAGCTTGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAAGCTAATGGGATTTGTCTCGAGACAAATCCCATTAGCTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAAGCTAATGGGATTTGTCTCGAGACAAATCCCATTAGCTTGACG-3'

Other clones with same target sequence:

  1. TRCN0000317320