Construct: shRNA TRCN0000124814
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_053182.2-2564s1c1
- DNA Barcode:
- CCTTCCAAACAGAGCCTTAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pag1 (94212)
- Gene Description:
- phosphoprotein associated with glycosphingolipid microdomains 1
- Transcript:
- RefSeq NM_053182.2 (NON-CURRENT)
- Match location:
- Position 2564 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000124814)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTTCCAAACAGAGCCTTAAT
- Hairpin Sequence:
-
5'-CCGG-CCTTCCAAACAGAGCCTTAAT-CTCGAG-ATTAAGGCTCTGTTTGGAAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTTCCAAACAGAGCCTTAATCTCGAGATTAAGGCTCTGTTTGGAAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTTCCAAACAGAGCCTTAATCTCGAGATTAAGGCTCTGTTTGGAAGG-3'
Other clones with same target sequence:
(none)
