Construct: shRNA TRCN0000124888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025822.2-817s1c1
DNA Barcode:
GCAGACATTCAGATCAAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rsrc1 (66880)
Gene Description:
arginine/serine-rich coiled-coil 1
Transcript:
RefSeq NM_025822.2 (NON-CURRENT)
Match location:
Position 817 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACATTCAGATCAAGTAA
Hairpin Sequence:
5'-CCGG-GCAGACATTCAGATCAAGTAA-CTCGAG-TTACTTGATCTGAATGTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACATTCAGATCAAGTAACTCGAGTTACTTGATCTGAATGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACATTCAGATCAAGTAACTCGAGTTACTTGATCTGAATGTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000128437
  2. TRCN0000281011