Construct: shRNA TRCN0000124959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177307.2-1921s1c1
DNA Barcode:
GCTTTGTTTGTAGAGGAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4f39 (320997)
Gene Description:
cytochrome P450, family 4, subfamily f, polypeptide 39
Transcript:
RefSeq NM_177307.2 (NON-CURRENT)
Match location:
Position 1921 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGTTTGTAGAGGAGTAT
Hairpin Sequence:
5'-CCGG-GCTTTGTTTGTAGAGGAGTAT-CTCGAG-ATACTCCTCTACAAACAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGTTTGTAGAGGAGTATCTCGAGATACTCCTCTACAAACAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGTTTGTAGAGGAGTATCTCGAGATACTCCTCTACAAACAAAGC-3'

Other clones with same target sequence:

(none)