Construct: shRNA TRCN0000124960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177307.2-848s1c1
DNA Barcode:
CCAGGAGAGAATGAGCGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4f39 (320997)
Gene Description:
cytochrome P450, family 4, subfamily f, polypeptide 39
Transcript:
RefSeq NM_177307.2 (NON-CURRENT)
Match location:
Position 848 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAGAGAATGAGCGATTA
Hairpin Sequence:
5'-CCGG-CCAGGAGAGAATGAGCGATTA-CTCGAG-TAATCGCTCATTCTCTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAGAGAATGAGCGATTACTCGAGTAATCGCTCATTCTCTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAGAGAATGAGCGATTACTCGAGTAATCGCTCATTCTCTCCTGG-3'

Other clones with same target sequence:

(none)