Construct: shRNA TRCN0000124967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026362.1-393s1c1
DNA Barcode:
CTTTAGCAACTGGAGCACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plgrkt (67759)
Gene Description:
plasminogen receptor, C-terminal lysine transmembrane protein
Transcript:
RefSeq NM_026362.1 (NON-CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTAGCAACTGGAGCACTAA
Hairpin Sequence:
5'-CCGG-CTTTAGCAACTGGAGCACTAA-CTCGAG-TTAGTGCTCCAGTTGCTAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTAGCAACTGGAGCACTAACTCGAGTTAGTGCTCCAGTTGCTAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTAGCAACTGGAGCACTAACTCGAGTTAGTGCTCCAGTTGCTAAAG-3'

Other clones with same target sequence:

  1. TRCN0000309561