Construct: shRNA TRCN0000124992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178146.2-821s1c1
DNA Barcode:
GTGGGATTAGTTTCAATGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tm9sf3 (107358)
Gene Description:
transmembrane 9 superfamily member 3
Transcript:
RefSeq NM_178146.2 (NON-CURRENT)
Match location:
Position 821 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGGATTAGTTTCAATGATT
Hairpin Sequence:
5'-CCGG-GTGGGATTAGTTTCAATGATT-CTCGAG-AATCATTGAAACTAATCCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGGATTAGTTTCAATGATTCTCGAGAATCATTGAAACTAATCCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGGATTAGTTTCAATGATTCTCGAGAATCATTGAAACTAATCCCAC-3'

Other clones with same target sequence:

  1. TRCN0000311970