Construct: shRNA TRCN0000125024

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025581.1-5161s1c1
DNA Barcode:
CCCGCATCTTATGAGGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnc2 (268345)
Gene Description:
potassium voltage gated channel, Shaw-related subfamily, member 2
Transcript:
RefSeq NM_001025581.1 (CURRENT)
Match location:
Position 5161 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125024)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCATCTTATGAGGCATTT
Hairpin Sequence:
5'-CCGG-CCCGCATCTTATGAGGCATTT-CTCGAG-AAATGCCTCATAAGATGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCATCTTATGAGGCATTTCTCGAGAAATGCCTCATAAGATGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCATCTTATGAGGCATTTCTCGAGAAATGCCTCATAAGATGCGGG-3'

Other clones with same target sequence:

(none)