Construct: shRNA TRCN0000125040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027543.3-1407s1c1
DNA Barcode:
CCTGCTTAACAAGGACCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr173 (70771)
Gene Description:
G-protein coupled receptor 173
Transcript:
RefSeq NM_027543.3 (NON-CURRENT)
Match location:
Position 1407 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTTAACAAGGACCTCAA
Hairpin Sequence:
5'-CCGG-CCTGCTTAACAAGGACCTCAA-CTCGAG-TTGAGGTCCTTGTTAAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTTAACAAGGACCTCAACTCGAGTTGAGGTCCTTGTTAAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTTAACAAGGACCTCAACTCGAGTTGAGGTCCTTGTTAAGCAGG-3'

Other clones with same target sequence:

(none)