Construct: shRNA TRCN0000125083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007791.2-155s1c1
DNA Barcode:
GAGGGCAACAGCTTCCATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csrp1 (13007)
Gene Description:
cysteine and glycine-rich protein 1
Transcript:
RefSeq NM_007791.2 (NON-CURRENT)
Match location:
Position 155 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGCAACAGCTTCCATAAA
Hairpin Sequence:
5'-CCGG-GAGGGCAACAGCTTCCATAAA-CTCGAG-TTTATGGAAGCTGTTGCCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGCAACAGCTTCCATAAACTCGAGTTTATGGAAGCTGTTGCCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGCAACAGCTTCCATAAACTCGAGTTTATGGAAGCTGTTGCCCTC-3'

Other clones with same target sequence:

  1. TRCN0000351668