Construct: shRNA TRCN0000125099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199322.1-4987s1c1
DNA Barcode:
GTCCAGTTTGTACTGTCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dot1l (208266)
Gene Description:
DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Transcript:
RefSeq NM_199322.1 (CURRENT)
Match location:
Position 4987 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCAGTTTGTACTGTCAATA
Hairpin Sequence:
5'-CCGG-GTCCAGTTTGTACTGTCAATA-CTCGAG-TATTGACAGTACAAACTGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCAGTTTGTACTGTCAATACTCGAGTATTGACAGTACAAACTGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCAGTTTGTACTGTCAATACTCGAGTATTGACAGTACAAACTGGAC-3'

Other clones with same target sequence:

(none)