Construct: shRNA TRCN0000125099
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_199322.1-4987s1c1
- DNA Barcode:
- GTCCAGTTTGTACTGTCAATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Dot1l (208266)
- Gene Description:
- DOT1-like, histone H3 methyltransferase (S. cerevisiae)
- Transcript:
- RefSeq NM_199322.1 (CURRENT)
- Match location:
- Position 4987 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000125099)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTCCAGTTTGTACTGTCAATA
- Hairpin Sequence:
-
5'-CCGG-GTCCAGTTTGTACTGTCAATA-CTCGAG-TATTGACAGTACAAACTGGAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTCCAGTTTGTACTGTCAATACTCGAGTATTGACAGTACAAACTGGACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTCCAGTTTGTACTGTCAATACTCGAGTATTGACAGTACAAACTGGAC-3'
Other clones with same target sequence:
(none)
