Construct: shRNA TRCN0000125341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028012.1-972s1c1
DNA Barcode:
GTCTTTAGAAACCCTGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrcc4 (108138)
Gene Description:
X-ray repair complementing defective repair in Chinese hamster cells 4
Transcript:
RefSeq NM_028012.1 (NON-CURRENT)
Match location:
Position 972 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTTAGAAACCCTGAGAAA
Hairpin Sequence:
5'-CCGG-GTCTTTAGAAACCCTGAGAAA-CTCGAG-TTTCTCAGGGTTTCTAAAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTTAGAAACCCTGAGAAACTCGAGTTTCTCAGGGTTTCTAAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTTAGAAACCCTGAGAAACTCGAGTTTCTCAGGGTTTCTAAAGAC-3'

Other clones with same target sequence:

(none)