Construct: shRNA TRCN0000125351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178721.2-1146s1c1
DNA Barcode:
GCTGATATAAGATGGTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cadm2 (239857)
Gene Description:
cell adhesion molecule 2
Transcript:
RefSeq NM_178721.2 (NON-CURRENT)
Match location:
Position 1146 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATATAAGATGGTTCAAA
Hairpin Sequence:
5'-CCGG-GCTGATATAAGATGGTTCAAA-CTCGAG-TTTGAACCATCTTATATCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATATAAGATGGTTCAAACTCGAGTTTGAACCATCTTATATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATATAAGATGGTTCAAACTCGAGTTTGAACCATCTTATATCAGC-3'

Other clones with same target sequence:

(none)