Construct: shRNA TRCN0000125454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026047.3-2532s1c1
DNA Barcode:
CCTGTGTTTCTTAGAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf219 (72486)
Gene Description:
ring finger protein 219
Transcript:
RefSeq NM_026047.3 (NON-CURRENT)
Match location:
Position 2532 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTTTCTTAGAACATTT
Hairpin Sequence:
5'-CCGG-CCTGTGTTTCTTAGAACATTT-CTCGAG-AAATGTTCTAAGAAACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTTTCTTAGAACATTTCTCGAGAAATGTTCTAAGAAACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTTTCTTAGAACATTTCTCGAGAAATGTTCTAAGAAACACAGG-3'

Other clones with same target sequence:

(none)