Construct: shRNA TRCN0000125464

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133710.1-2506s1c1
DNA Barcode:
GCAGTCAACATCAGAGCCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctdspl (69274)
Gene Description:
CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like
Transcript:
RefSeq NM_133710.1 (NON-CURRENT)
Match location:
Position 2506 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125464)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCAACATCAGAGCCTAA
Hairpin Sequence:
5'-CCGG-GCAGTCAACATCAGAGCCTAA-CTCGAG-TTAGGCTCTGATGTTGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCAACATCAGAGCCTAACTCGAGTTAGGCTCTGATGTTGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCAACATCAGAGCCTAACTCGAGTTAGGCTCTGATGTTGACTGC-3'

Other clones with same target sequence:

(none)