Construct: shRNA TRCN0000125469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025944.2-1821s1c1
DNA Barcode:
GCGAGACTTGAATTAGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem246 (67063)
Gene Description:
transmembrane protein 246
Transcript:
RefSeq NM_025944.2 (NON-CURRENT)
Match location:
Position 1821 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGACTTGAATTAGGATTT
Hairpin Sequence:
5'-CCGG-GCGAGACTTGAATTAGGATTT-CTCGAG-AAATCCTAATTCAAGTCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGACTTGAATTAGGATTTCTCGAGAAATCCTAATTCAAGTCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGACTTGAATTAGGATTTCTCGAGAAATCCTAATTCAAGTCTCGC-3'

Other clones with same target sequence:

(none)