Construct: shRNA TRCN0000125485
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172684.2-1598s1c1
- DNA Barcode:
- CCAGACTTCTTGGACTACTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rsbn1 (229675)
- Gene Description:
- rosbin, round spermatid basic protein 1
- Transcript:
- RefSeq NM_172684.2 (CURRENT)
- Match location:
- Position 1598 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000125485)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGACTTCTTGGACTACTTT
- Hairpin Sequence:
-
5'-CCGG-CCAGACTTCTTGGACTACTTT-CTCGAG-AAAGTAGTCCAAGAAGTCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGACTTCTTGGACTACTTTCTCGAGAAAGTAGTCCAAGAAGTCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGACTTCTTGGACTACTTTCTCGAGAAAGTAGTCCAAGAAGTCTGG-3'
Other clones with same target sequence:
-
TRCN0000136557
