Construct: shRNA TRCN0000125491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021050.1-1208s1c1
DNA Barcode:
CCGTACAGATATGGTATGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cftr (12638)
Gene Description:
cystic fibrosis transmembrane conductance regulator
Transcript:
RefSeq NM_021050.1 (NON-CURRENT)
Match location:
Position 1208 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTACAGATATGGTATGATT
Hairpin Sequence:
5'-CCGG-CCGTACAGATATGGTATGATT-CTCGAG-AATCATACCATATCTGTACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTACAGATATGGTATGATTCTCGAGAATCATACCATATCTGTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTACAGATATGGTATGATTCTCGAGAATCATACCATATCTGTACGG-3'

Other clones with same target sequence:

(none)