Construct: shRNA TRCN0000125508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133973.1-896s1c1
DNA Barcode:
CGACTCTTTACCCTGATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cog4 (102339)
Gene Description:
component of oligomeric golgi complex 4
Transcript:
RefSeq NM_133973.1 (NON-CURRENT)
Match location:
Position 896 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTCTTTACCCTGATCAAA
Hairpin Sequence:
5'-CCGG-CGACTCTTTACCCTGATCAAA-CTCGAG-TTTGATCAGGGTAAAGAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTCTTTACCCTGATCAAACTCGAGTTTGATCAGGGTAAAGAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTCTTTACCCTGATCAAACTCGAGTTTGATCAGGGTAAAGAGTCG-3'

Other clones with same target sequence:

(none)