Construct: shRNA TRCN0000125517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177708.3-1613s1c1
DNA Barcode:
TCTGGGAAAGAGCTTACCGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtn4rl1 (237847)
Gene Description:
reticulon 4 receptor-like 1
Transcript:
RefSeq NM_177708.3 (NON-CURRENT)
Match location:
Position 1613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGGGAAAGAGCTTACCGAA
Hairpin Sequence:
5'-CCGG-TCTGGGAAAGAGCTTACCGAA-CTCGAG-TTCGGTAAGCTCTTTCCCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGGGAAAGAGCTTACCGAACTCGAGTTCGGTAAGCTCTTTCCCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGGGAAAGAGCTTACCGAACTCGAGTTCGGTAAGCTCTTTCCCAGA-3'

Other clones with same target sequence:

(none)