Construct: shRNA TRCN0000125611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172266.1-1132s1c1
DNA Barcode:
CCTCAGCAACATGTGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lpgat1 (226856)
Gene Description:
lysophosphatidylglycerol acyltransferase 1
Transcript:
RefSeq NM_172266.1 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGCAACATGTGGATATT
Hairpin Sequence:
5'-CCGG-CCTCAGCAACATGTGGATATT-CTCGAG-AATATCCACATGTTGCTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGCAACATGTGGATATTCTCGAGAATATCCACATGTTGCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGCAACATGTGGATATTCTCGAGAATATCCACATGTTGCTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000323923