Construct: shRNA TRCN0000125630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029673.2-2035s1c1
DNA Barcode:
CCCGTTTCTATGCTGTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Immt (76614)
Gene Description:
inner membrane protein, mitochondrial
Transcript:
RefSeq NM_029673.2 (NON-CURRENT)
Match location:
Position 2035 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTTTCTATGCTGTTCAAA
Hairpin Sequence:
5'-CCGG-CCCGTTTCTATGCTGTTCAAA-CTCGAG-TTTGAACAGCATAGAAACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTTTCTATGCTGTTCAAACTCGAGTTTGAACAGCATAGAAACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTTTCTATGCTGTTCAAACTCGAGTTTGAACAGCATAGAAACGGG-3'

Other clones with same target sequence:

  1. TRCN0000323849