Construct: shRNA TRCN0000125681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028990.2-2071s1c1
DNA Barcode:
GCGAAGACAGTAGATATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem168 (101118)
Gene Description:
transmembrane protein 168
Transcript:
RefSeq NM_028990.2 (NON-CURRENT)
Match location:
Position 2071 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAAGACAGTAGATATTGAA
Hairpin Sequence:
5'-CCGG-GCGAAGACAGTAGATATTGAA-CTCGAG-TTCAATATCTACTGTCTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAAGACAGTAGATATTGAACTCGAGTTCAATATCTACTGTCTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAAGACAGTAGATATTGAACTCGAGTTCAATATCTACTGTCTTCGC-3'

Other clones with same target sequence:

  1. TRCN0000323942